How to determine reaction order based on graph?

You can also determine the reaction order of an enzyme catalyzed reaction based on a graph of initial reaction rate vs. enzyme concentration. The best fit line to the data should give the reaction order. The line with a negative slope gives first order while a line with a positive slope gives second order.

How to determine reaction order based on slope?

If the graph has a straight line appearance, the reaction can happen with either first-order or zero-order kinetics. A straight line means that the rate of reaction does not change with the reaction time. This is because the rate-limiting step does not depend on the time. If there is a downward or upward curve to the graph, the reaction will not happen with first-order kinetics. This means that the rate of reaction decreases or increases with time.

How to determine reaction order based on initial rate?

If the graphs of the first-order, second-order, and zero-order reaction rates are linear, the reaction is likely first-order. If the graphs are nonlinear, the reaction is likely second-order. If the reaction rate increases or decreases rapidly, it is likely first-order. If the reaction rate saturates, it is likely zero-order.

How to determine the order of reaction based on initial rate?

The simplest way to determine the order of reaction is by initial rate graphs. A typical initial rate graph is a line that plots the initial rate of reaction as a function of substrate concentration. The initial rate is the rate of change of reaction at time zero. For first-order reactions, the initial rate increases linearly with substrate concentration, giving a straight line. For second-order reactions, the initial rate increases with the square root of the substrate concentration, giving a curve. That’s why

How to determine the rate of a reaction based on initial rate?

The initial rate approach is the most straightforward way to determine the reaction order. The rate of reaction is dependent on the concentration of the reactants; thus, the initial rate is dependent on the concentration of the reactant at the beginning of the reaction. Since the rate of reaction is dependent on the initial concentration of the reactant, the reaction order can be determined by taking the initial rate of reaction at varying initial concentrations of the reactant. A reaction graph of the initial rate of reaction is drawn.