How to determine reaction rate experimentally?

To determine the reaction rate of an enzymatic reaction, you need to measure the rate of product increase as a function of time. To do this, you need to start the reaction in a certain volume and measure the product level every minute for a sufficient period of time. You can use a simple beaker with a magnetic stirrer to do this. The beaker should have enough space to allow for the enzyme solution to reach the maximum possible reaction rate.

How to determine the reaction rate experimentally?

To determine the reaction rate, you will need to carry out an experiment that measures the rate at which the product is formed and the amount of reactant remaining at each point in time. The simplest way to do this is to start with a known amount of the reactant, add the catalyst then measure the amount of the product that forms at each time point. You can use a simple beaker and a stopwatch to track the amount of time it takes for the reaction to occur at a

How to determine the rate of reaction experimentally?

You can determine the rate of reaction in experiments in two ways: by determining the amount of products generated per unit time (product kinetic method) or by determining the amount of substrate consumed per unit time (substrate kinetic method). Both methods can also be applied to the reverse reaction, i.e., for the decomposition of the product.

How to determine reaction rate quantum mechanically?

The reaction rate can be calculated using the principle of the quantum tunnelling. Quantum tunnelling is the process in which a particle can pass through a barrier, as shown in the figure below. If the energy of the particle is lower than the height of the barrier, the particle can pass through it.

How to determine the rate of reaction with diazo methane?

Now we will perform an actual reaction with diazo methane. In this example, we will use the reaction between diazonium salts and ketones or aldehydes to form diazo compounds. We will use diazo methane as a diazonium salt because it is relatively easy to handle. Add 100 mL of diazotized methane to a flask containing a solution of butyl ketone (4 mL, 0.05 g) or 3-pentanone